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pericyte media (pm  (ScienCell)

 
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    Structured Review

    ScienCell pericyte media (pm
    A) Schematic showing the process of generating the 3D NVU system in the MIMETAS microfluidic system. B) Brightfield images showing HBMEC and rBMEC only tubules, as well as their co-cultures with primary astrocytes (AC) and primary <t>pericytes</t> (PC) or iPSC-derived astrocytes (iACs) and pericytes (iPCs), respectively. C) Representative immunofluorescence staining images for rBMECs showing endothelial marker CD31 and VE-cadherin (top panel) and junctional expression of TJ markers ZO-1 and Claudin-5 (bottom panel) in tubules formed inside 3D microfluidic channels; scale bar = 50 µm D) 3D view of representative immunofluorescence staining for MIMETAS microfluidic culture showing all three channels with PDGFRβ + pericytes and S100β + astrocytes in brain channel and rBMEC tubule in the blood channel. E) Close up view of representative immunofluorescence staining showing TJ marker ZO-1 for rBMECs, S100β for astrocytes, and PDGFRβ for pericytes in the blood channel; cross sectional view shows tubule with open lumen. F) Representative image showing differential diffusion of fluorescent tracer from leaky and tight barriers in 3D microfluidic culture. G-H) Permeability measurement of primary HBMEC tubules, HBMEC tubules with ACs <t>and</t> <t>PCs,</t> rBMEC tubules, and rBMEC tubules with iACs and iPCs in the middle ECM channel using large 70 kDa dextran and small ∼900Da Biocytin tracer over a course of 45 minutes. N=36-41 NVU chips. Data was analyzed using two-way ANOVA and presented as mean±SD for 3 independent experiments, ns, **<0.01, ****<0.0001. I) 3D TEER measurement comparing primary HBMEC tubules, HBMEC tubules with ACs and PCs, rBMEC tubules, and rBMEC tubules with iACs and iPCs; each point represents one individual NVU chip. N = 30-48. Data is presented as mean±SD, and statistical comparisons were made between each group using one-way ANOVA, ****<0.0001, *<0.05.
    Pericyte Media (Pm, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pericyte+media+%28pm/bio_rxiv__2024__04__03__588012-162-4-7?v=ScienCell
    Average 90 stars, based on 1 article reviews
    pericyte media (pm - by Bioz Stars, 2026-08
    90/100 stars

    Images

    1) Product Images from "Generation of hiPSC-derived brain microvascular endothelial cells using a combination of directed differentiation and transcriptional reprogramming strategies"

    Article Title: Generation of hiPSC-derived brain microvascular endothelial cells using a combination of directed differentiation and transcriptional reprogramming strategies

    Journal: bioRxiv

    doi: 10.1101/2024.04.03.588012

    A) Schematic showing the process of generating the 3D NVU system in the MIMETAS microfluidic system. B) Brightfield images showing HBMEC and rBMEC only tubules, as well as their co-cultures with primary astrocytes (AC) and primary pericytes (PC) or iPSC-derived astrocytes (iACs) and pericytes (iPCs), respectively. C) Representative immunofluorescence staining images for rBMECs showing endothelial marker CD31 and VE-cadherin (top panel) and junctional expression of TJ markers ZO-1 and Claudin-5 (bottom panel) in tubules formed inside 3D microfluidic channels; scale bar = 50 µm D) 3D view of representative immunofluorescence staining for MIMETAS microfluidic culture showing all three channels with PDGFRβ + pericytes and S100β + astrocytes in brain channel and rBMEC tubule in the blood channel. E) Close up view of representative immunofluorescence staining showing TJ marker ZO-1 for rBMECs, S100β for astrocytes, and PDGFRβ for pericytes in the blood channel; cross sectional view shows tubule with open lumen. F) Representative image showing differential diffusion of fluorescent tracer from leaky and tight barriers in 3D microfluidic culture. G-H) Permeability measurement of primary HBMEC tubules, HBMEC tubules with ACs and PCs, rBMEC tubules, and rBMEC tubules with iACs and iPCs in the middle ECM channel using large 70 kDa dextran and small ∼900Da Biocytin tracer over a course of 45 minutes. N=36-41 NVU chips. Data was analyzed using two-way ANOVA and presented as mean±SD for 3 independent experiments, ns, **<0.01, ****<0.0001. I) 3D TEER measurement comparing primary HBMEC tubules, HBMEC tubules with ACs and PCs, rBMEC tubules, and rBMEC tubules with iACs and iPCs; each point represents one individual NVU chip. N = 30-48. Data is presented as mean±SD, and statistical comparisons were made between each group using one-way ANOVA, ****<0.0001, *<0.05.
    Figure Legend Snippet: A) Schematic showing the process of generating the 3D NVU system in the MIMETAS microfluidic system. B) Brightfield images showing HBMEC and rBMEC only tubules, as well as their co-cultures with primary astrocytes (AC) and primary pericytes (PC) or iPSC-derived astrocytes (iACs) and pericytes (iPCs), respectively. C) Representative immunofluorescence staining images for rBMECs showing endothelial marker CD31 and VE-cadherin (top panel) and junctional expression of TJ markers ZO-1 and Claudin-5 (bottom panel) in tubules formed inside 3D microfluidic channels; scale bar = 50 µm D) 3D view of representative immunofluorescence staining for MIMETAS microfluidic culture showing all three channels with PDGFRβ + pericytes and S100β + astrocytes in brain channel and rBMEC tubule in the blood channel. E) Close up view of representative immunofluorescence staining showing TJ marker ZO-1 for rBMECs, S100β for astrocytes, and PDGFRβ for pericytes in the blood channel; cross sectional view shows tubule with open lumen. F) Representative image showing differential diffusion of fluorescent tracer from leaky and tight barriers in 3D microfluidic culture. G-H) Permeability measurement of primary HBMEC tubules, HBMEC tubules with ACs and PCs, rBMEC tubules, and rBMEC tubules with iACs and iPCs in the middle ECM channel using large 70 kDa dextran and small ∼900Da Biocytin tracer over a course of 45 minutes. N=36-41 NVU chips. Data was analyzed using two-way ANOVA and presented as mean±SD for 3 independent experiments, ns, **<0.01, ****<0.0001. I) 3D TEER measurement comparing primary HBMEC tubules, HBMEC tubules with ACs and PCs, rBMEC tubules, and rBMEC tubules with iACs and iPCs; each point represents one individual NVU chip. N = 30-48. Data is presented as mean±SD, and statistical comparisons were made between each group using one-way ANOVA, ****<0.0001, *<0.05.

    Techniques Used: Derivative Assay, Immunofluorescence, Staining, Marker, Expressing, Diffusion-based Assay, Permeability



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    A) Schematic showing the process of generating the 3D NVU system in the MIMETAS microfluidic system. B) Brightfield images showing HBMEC and rBMEC only tubules, as well as their co-cultures with primary astrocytes (AC) and primary <t>pericytes</t> (PC) or iPSC-derived astrocytes (iACs) and pericytes (iPCs), respectively. C) Representative immunofluorescence staining images for rBMECs showing endothelial marker CD31 and VE-cadherin (top panel) and junctional expression of TJ markers ZO-1 and Claudin-5 (bottom panel) in tubules formed inside 3D microfluidic channels; scale bar = 50 µm D) 3D view of representative immunofluorescence staining for MIMETAS microfluidic culture showing all three channels with PDGFRβ + pericytes and S100β + astrocytes in brain channel and rBMEC tubule in the blood channel. E) Close up view of representative immunofluorescence staining showing TJ marker ZO-1 for rBMECs, S100β for astrocytes, and PDGFRβ for pericytes in the blood channel; cross sectional view shows tubule with open lumen. F) Representative image showing differential diffusion of fluorescent tracer from leaky and tight barriers in 3D microfluidic culture. G-H) Permeability measurement of primary HBMEC tubules, HBMEC tubules with ACs <t>and</t> <t>PCs,</t> rBMEC tubules, and rBMEC tubules with iACs and iPCs in the middle ECM channel using large 70 kDa dextran and small ∼900Da Biocytin tracer over a course of 45 minutes. N=36-41 NVU chips. Data was analyzed using two-way ANOVA and presented as mean±SD for 3 independent experiments, ns, **<0.01, ****<0.0001. I) 3D TEER measurement comparing primary HBMEC tubules, HBMEC tubules with ACs and PCs, rBMEC tubules, and rBMEC tubules with iACs and iPCs; each point represents one individual NVU chip. N = 30-48. Data is presented as mean±SD, and statistical comparisons were made between each group using one-way ANOVA, ****<0.0001, *<0.05.
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    A) Schematic showing the process of generating the 3D NVU system in the MIMETAS microfluidic system. B) Brightfield images showing HBMEC and rBMEC only tubules, as well as their co-cultures with primary astrocytes (AC) and primary <t>pericytes</t> (PC) or iPSC-derived astrocytes (iACs) and pericytes (iPCs), respectively. C) Representative immunofluorescence staining images for rBMECs showing endothelial marker CD31 and VE-cadherin (top panel) and junctional expression of TJ markers ZO-1 and Claudin-5 (bottom panel) in tubules formed inside 3D microfluidic channels; scale bar = 50 µm D) 3D view of representative immunofluorescence staining for MIMETAS microfluidic culture showing all three channels with PDGFRβ + pericytes and S100β + astrocytes in brain channel and rBMEC tubule in the blood channel. E) Close up view of representative immunofluorescence staining showing TJ marker ZO-1 for rBMECs, S100β for astrocytes, and PDGFRβ for pericytes in the blood channel; cross sectional view shows tubule with open lumen. F) Representative image showing differential diffusion of fluorescent tracer from leaky and tight barriers in 3D microfluidic culture. G-H) Permeability measurement of primary HBMEC tubules, HBMEC tubules with ACs <t>and</t> <t>PCs,</t> rBMEC tubules, and rBMEC tubules with iACs and iPCs in the middle ECM channel using large 70 kDa dextran and small ∼900Da Biocytin tracer over a course of 45 minutes. N=36-41 NVU chips. Data was analyzed using two-way ANOVA and presented as mean±SD for 3 independent experiments, ns, **<0.01, ****<0.0001. I) 3D TEER measurement comparing primary HBMEC tubules, HBMEC tubules with ACs and PCs, rBMEC tubules, and rBMEC tubules with iACs and iPCs; each point represents one individual NVU chip. N = 30-48. Data is presented as mean±SD, and statistical comparisons were made between each group using one-way ANOVA, ****<0.0001, *<0.05.
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    Image Search Results


    A) Schematic showing the process of generating the 3D NVU system in the MIMETAS microfluidic system. B) Brightfield images showing HBMEC and rBMEC only tubules, as well as their co-cultures with primary astrocytes (AC) and primary pericytes (PC) or iPSC-derived astrocytes (iACs) and pericytes (iPCs), respectively. C) Representative immunofluorescence staining images for rBMECs showing endothelial marker CD31 and VE-cadherin (top panel) and junctional expression of TJ markers ZO-1 and Claudin-5 (bottom panel) in tubules formed inside 3D microfluidic channels; scale bar = 50 µm D) 3D view of representative immunofluorescence staining for MIMETAS microfluidic culture showing all three channels with PDGFRβ + pericytes and S100β + astrocytes in brain channel and rBMEC tubule in the blood channel. E) Close up view of representative immunofluorescence staining showing TJ marker ZO-1 for rBMECs, S100β for astrocytes, and PDGFRβ for pericytes in the blood channel; cross sectional view shows tubule with open lumen. F) Representative image showing differential diffusion of fluorescent tracer from leaky and tight barriers in 3D microfluidic culture. G-H) Permeability measurement of primary HBMEC tubules, HBMEC tubules with ACs and PCs, rBMEC tubules, and rBMEC tubules with iACs and iPCs in the middle ECM channel using large 70 kDa dextran and small ∼900Da Biocytin tracer over a course of 45 minutes. N=36-41 NVU chips. Data was analyzed using two-way ANOVA and presented as mean±SD for 3 independent experiments, ns, **<0.01, ****<0.0001. I) 3D TEER measurement comparing primary HBMEC tubules, HBMEC tubules with ACs and PCs, rBMEC tubules, and rBMEC tubules with iACs and iPCs; each point represents one individual NVU chip. N = 30-48. Data is presented as mean±SD, and statistical comparisons were made between each group using one-way ANOVA, ****<0.0001, *<0.05.

    Journal: bioRxiv

    Article Title: Generation of hiPSC-derived brain microvascular endothelial cells using a combination of directed differentiation and transcriptional reprogramming strategies

    doi: 10.1101/2024.04.03.588012

    Figure Lengend Snippet: A) Schematic showing the process of generating the 3D NVU system in the MIMETAS microfluidic system. B) Brightfield images showing HBMEC and rBMEC only tubules, as well as their co-cultures with primary astrocytes (AC) and primary pericytes (PC) or iPSC-derived astrocytes (iACs) and pericytes (iPCs), respectively. C) Representative immunofluorescence staining images for rBMECs showing endothelial marker CD31 and VE-cadherin (top panel) and junctional expression of TJ markers ZO-1 and Claudin-5 (bottom panel) in tubules formed inside 3D microfluidic channels; scale bar = 50 µm D) 3D view of representative immunofluorescence staining for MIMETAS microfluidic culture showing all three channels with PDGFRβ + pericytes and S100β + astrocytes in brain channel and rBMEC tubule in the blood channel. E) Close up view of representative immunofluorescence staining showing TJ marker ZO-1 for rBMECs, S100β for astrocytes, and PDGFRβ for pericytes in the blood channel; cross sectional view shows tubule with open lumen. F) Representative image showing differential diffusion of fluorescent tracer from leaky and tight barriers in 3D microfluidic culture. G-H) Permeability measurement of primary HBMEC tubules, HBMEC tubules with ACs and PCs, rBMEC tubules, and rBMEC tubules with iACs and iPCs in the middle ECM channel using large 70 kDa dextran and small ∼900Da Biocytin tracer over a course of 45 minutes. N=36-41 NVU chips. Data was analyzed using two-way ANOVA and presented as mean±SD for 3 independent experiments, ns, **<0.01, ****<0.0001. I) 3D TEER measurement comparing primary HBMEC tubules, HBMEC tubules with ACs and PCs, rBMEC tubules, and rBMEC tubules with iACs and iPCs; each point represents one individual NVU chip. N = 30-48. Data is presented as mean±SD, and statistical comparisons were made between each group using one-way ANOVA, ****<0.0001, *<0.05.

    Article Snippet: PCs were cultured in Pericyte Media (PM, ScienCell), and ACs were cultured in Astrocyte Media (AM, ScienCell).

    Techniques: Derivative Assay, Immunofluorescence, Staining, Marker, Expressing, Diffusion-based Assay, Permeability